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ERX11798559: RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ
1 ILLUMINA (Illumina NovaSeq 6000) run: 31.5M spots, 9.5G bases, 2.8Gb downloads

Design: RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ
Submitted by: EBI (European Bioinformatics Institute)
Study: RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ
PRJEB71814 • ERP156600 • All experiments • All runs
show Abstracthide Abstract
This study aims to identify combination treatments capable of inducing improved IO responses in lung tumours and thus, help guide decisions on the next combination arms for the HUDSON trial (post-IO). For that purpose, a lung tumour GEMM model was treated with either vehicle, PD-L1, ATR, ATR/PD-L1; Cisplatin/PD-L1/Ctla4 or VEGFR/PD-L1 and tumours collected for transcriptional profiling.
Sample: LUGEMM14 7-63
SAMEA115098483 • ERS17733230 • All experiments • All runs
Organism: Mus musculus
Library:
Name: LUGEMM14 7-63_p
Instrument: Illumina NovaSeq 6000
Strategy: AMPLICON
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Construction protocol: Samples were collected from GEMMs lung lesions and assessed by MRI quantification of combined lung tumour lesions per animal before & after treatment RNA was extracted using the RNeasy Mini Kit (Qiagen; #74104). RNA concentration was determined by Qubit Flex Fluorometer (Invitrogen), RNA purity was determined using a NanoDrop Eight (Thermo Scientific) and RNA integrity was measured using a 4200 Tapestation (Agilent) Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs; E7760L) or NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs; E7770L) as per manufactuer's guidelines. Ribosomal RNA was removed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs; E7490L) .
Runs: 1 run, 31.5M spots, 9.5G bases, 2.8Gb
Run# of Spots# of BasesSizePublished
ERR1242229331,549,5359.5G2.8Gb2024-01-30

ID:
31670413

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